What are the advantages and disadvantages of electron microscopy? Operation and analysis requires special training.Potential artifacts from sample preparation.Some cons of electron microscopes include:.What are the disadvantages of transmission electron microscope? And it cannot see smaller organelles like ribosomes. ![]() The main limitation of light microscopes are, that they have a lower resolution and cannot see beyond 200nm. What is a limitation of the light microscope explain why this is a limitation? This value may be approximately halved, with some inconvenience, using ultraviolet radiation of shorter wavelengths. Using an objective of NA 1.4, and green light of wavelength 500 nm, the resolution limit is ∼0.2 μm. The principal limitation of the light microscope is its resolving power. ![]() What is the limitation of light microscope?
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